FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007814

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007814
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1046211
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAAGT4553.6684013E-412.8310061
AGAGACA4700.00735529910.7529674
CTACAAT9803.496695E-58.935881
AGACTTT10357.519443E-58.3708384
CCTCAAT8400.0064940987.8188951
CCTCAAA11652.621536E-47.5168771
TCAGGTT9700.00309484367.4431432
CTTTCAC11200.00142217977.16732031
CCCGCAG13602.0197652E-46.9756811
CCACATT10850.0082837836.72593161
CCCCGGC13300.00119144736.58433251
CTTTAAT13500.00141059886.48678641
TCAGAAT13550.0016598646.3939622
CTTTTAT19801.2487091E-56.26564651
CTGCCGA16402.8862729E-46.22968861
CAAAGGA16703.6463304E-46.1177781
GATTGAA13100.0082255066.06159935
CATAAAT15600.0070946175.61356541
CTTTGTC15900.0087517345.50764941
Produced by FastQC (version 0.11.5)