FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007817

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007817
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1647716
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCAAT14551.0441363E-78.4826341
CTTAAAC7800.0034939538.377081
CCCTTCT12851.2533399E-57.90987631
CCCAGTT12255.9207596E-57.7046341
CTCAAAT14256.0111706E-67.6422491
CTCAGGC8700.0093129447.5104861
CCCTTAT10709.2551403E-47.46369171
CCCAGCT11903.5556464E-47.32114651
CTCGAAG11100.00136701137.19473031
CTTCGGA19552.0901098E-77.05588771
CTTGCAT12405.698616E-47.02593851
CTTCTGT23802.6993803E-97.01609851
CTTGATC10450.0060588146.9474991
CCCCATT11650.00227894426.85506441
CTTATAT21301.2815326E-76.817031
CTCAAAA22504.8430593E-86.7761281
CCCGCTT13009.7620365E-46.70166441
CTTCGAT17351.3107961E-56.6952261
CCCGATT10900.0091072266.66067551
GTCTTGT12150.00354332736.5729631
Produced by FastQC (version 0.11.5)