FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007828

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007828
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences569666
Sequences flagged as poor quality0
Sequence length100-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCACG2600.00185415816.6711836
CCCACGG3150.00646186313.8197841
CTTAAAT6250.0054346929.2868951
CTTCAAA10256.327151E-69.2019541
CTTCTTG12751.1336764E-57.96669861
CTTCTGT8750.0098389057.4626831
CCTTGAT10700.0076467946.78073551
AAACAAT12800.0064137136.2077344
AAAGATA18500.00152790575.46646644
Produced by FastQC (version 0.11.5)