FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007836

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007836
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences498768
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCAAAT3800.001467096613.3356841
CTCGCGG3850.001618929113.1624931
CCCGGCT4800.00841786210.5574171
GTTTGAA6650.0092042498.7090181
ATTTAAT6700.0097857368.6440261
CTTTGAT7750.00337459828.4070121
CTTTCAA8659.728546E-48.3692151
CTTCCAG8700.0095304547.48900461
TTTTCCG12800.00696388776.15834147
TTTTTCC16150.00200594085.76893236
Produced by FastQC (version 0.11.5)