FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007854

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007854
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1391590
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCAAAC6954.827234E-813.4292541
TGTGTTG7450.0026176528.6485723
CTCGGAG6650.0098698288.6369751
CTCGAAC8050.00513352738.0267541
CTTCGAG8600.0092787987.51341631
TTCGATT12150.0040832676.4842832
CAGAGAT13550.00182692036.3401583
AACATCG12500.0055175566.2997785
TTGACTT12900.00764878336.10465533
CACAGAT13150.0093279555.9885973
CTTCAAT17950.00113105655.5995991
CCTCGCC15750.0094419225.47008471
AATGCAG21403.3603227E-45.3524025
Produced by FastQC (version 0.11.5)