FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007859

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007859
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1089091
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTCT4251.6716513E-920.260819
ACACGTC4756.6520442E-918.1280948
CACACGT4609.1011316E-817.159297
AGCACAC6352.3578468E-713.5603865
GAGCACA6904.4157787E-813.5200624
AGAGCAC6908.792971E-611.4400533
GCACACG7551.9173167E-611.4050926
CCCAAAA11051.796194E-58.4746881
CCACAAT10800.0089808816.66989331
CTTCCAC14400.00337018966.00290351
ATTCCGC14600.0041034525.89783868
AAAGATT17357.379397E-45.79045874
AAGATTT19354.8784085E-45.5625615
CAAAAAA28304.0579507E-65.34534171
ATTATGA18300.0082742375.11722951
Produced by FastQC (version 0.11.5)