FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007871

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007871
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences568448
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCAAT2003.1484597E-421.7009051
TAGTCCG1850.005253229319.4574999
CTTACAG4400.004433562511.5080571
CCTTCGA4800.00847005110.5490511
ATTTAAA7954.2340296E-49.0989121
CTTTAAA8155.426696E-48.8756261
CTCAAAA7550.00268410528.6228761
CTTCAAT8508.247396E-48.51015951
CTTCAAA9452.5306555E-48.4201051
CTTTTTT18104.6071727E-77.19367071
TTTAAAA10700.0081656016.7348112