FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007874

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007874
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2098469
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAAAT7350.00207692018.87237551
CTCAGAT9201.865635E-48.66342451
CTCCGTC23608.4799285E-76.1404861
CTCCAAC20151.8311888E-56.11305951
CCCAGCT19406.9185175E-55.97589541
CTCAAAT16000.00158203135.88719131
CTCTGTT15050.00516754445.77736041
CCCAAAT16550.00236740525.6915441
CTCACCG20902.0219653E-45.54700371
GTATTAT23854.0419123E-55.4685081
AACTTGT17500.004910625.3510246
CTCCGCT19000.0020662985.338991
CTCTTCT24606.603555E-55.3017851
CTTTAAT21903.9337727E-45.2937161
CTTCAGC33104.1166095E-75.25373271
CCTCGAT18000.00637877455.23305851
CATTCGT18300.00773855675.14727071
CCACGAT18400.0082464885.1192961
TTATAAC18550.0097062915.0481369