FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007877

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007877
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences644185
Sequences flagged as poor quality0
Sequence length100-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGCTC5450.029.1799131
ACACTCT3403.6379788E-1227.6389961
ACGCTCT6250.026.4837272
TCCCTAC2808.883944E-925.6943939
CGCTCTT7200.023.9795723
TCCGATC7400.023.3332869
GCTCTTC9700.017.799274
TTCCCTA5051.3709723E-817.0956768
CTTCCGA10150.017.0114617
CACTCTT5205.919326E-613.839742
CTCTTCC12650.013.6484535
TTCCGAT12800.013.4895578
TCTTCCG16200.011.545726
GTACAAT6304.0629413E-511.4740631
CACGACG2850.003048374310.0966624
CCGATCT6553.8357757E-79.8854769
GTTTAAA6450.00725933868.965781
CTCCGGT7350.00211786438.851421
TTTCCCT10951.62387E-58.5413337
AACGATA7700.0033723468.40842153
Produced by FastQC (version 0.11.5)