FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007881

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007881
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1068750
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCGGG10407.545859E-79.7373651
CTTCGAT9500.0024775337.61417961
CAACGAT11050.00135384727.200741
CTTTGAT17151.5206206E-67.1702041
CTTAAAA15701.839649E-46.45022871
CAACAAT12900.0068682486.1680761
AAAGGTT13100.0081968066.06367544
CGAAGAA17256.190179E-45.8706431
TCAGAAT14850.00462385645.8348372
CTTTGGC15000.00506861835.78677651
CTCGCCG16700.00268511125.6308461
TATGATT16800.00294365455.58737662
CTTTTAT21904.0211887E-45.28472761
Produced by FastQC (version 0.11.5)