FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007888

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007888
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences806279
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAAAT5952.8613873E-411.0097231
CCCGCCA7100.00145243179.2264571
CTCACCG7200.00164990499.0983121
CCCAGCG8104.8023835E-48.9859881
GATTTAC8357.2811E-48.6185355
CAAAGCA11201.9526668E-47.71051034
CTTCAAT13802.4916584E-46.8566991
CTTCATT14200.0025637116.15097141
CTTTAAT14300.00277338186.1079581
CTTCCAG14350.00288387626.08667561
GTTCTTT15750.001237716.00777441
CTTCTTT32103.311834E-85.66873031
CTGCTTT19950.00358592235.10782431
CATTTTC22556.5253605E-45.1061485
CTTTTTA18650.0091330825.07358931
Produced by FastQC (version 0.11.5)