FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007891

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007891
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1732359
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGAAT6657.8560587E-49.8688031
CTCGGTC6100.00464453049.46773
CTTAATT10951.3670196E-69.323041
TTAACCT6200.0053104329.3149963
CTCAACT7853.4511127E-49.2891061
CTCCCAC10609.124464E-68.9429561
CCTAAAG6650.0086931358.7722691
CCCAGAT15202.1422238E-88.6352021
CTCCTAT11302.0435713E-58.38896751
CTCAGGA9602.7592087E-48.3553571
CTCCCGT11352.160105E-58.3520121
CTCAGAA12054.5681132E-57.86683231
CCCCAAT9500.00229105357.67573551
CTCAAAA22004.7293724E-107.62340071
CTCAAAT15502.2927597E-67.5271721
CTTTGAT29900.07.31633661
CTCAAGA11100.0013051617.2262551
ATCGAAT12154.2934393E-47.20192431
CCTCGAT13201.4143459E-47.18146471
CTTAAAT14606.4204854E-56.99227951