FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007913

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007913
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences813218
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAAGC7155.4649718E-813.3004981
CTTCCGT8251.8462742E-913.3004961
CTTAGAA4450.004438868711.5071731
CCCGCAT5550.001900716610.54453851
CTTTAAG7250.00167875319.0810291
CTCCAAC6500.0070172769.0034131
CATAGAA8205.163074E-48.9210651
CAACGAT7500.00228477788.7783281
CTTATCT9953.914385E-48.0872371
ACAATCT9954.4894527E-47.98477843
CTCCATT8350.00602073557.8847261
CAACAAT12607.6307886E-57.54750441
CTTCATT11000.00114416057.31527331
CTTTATT17601.912169E-67.06588941
TTTTGCT15201.229722E-46.652397
CTTTTCT16554.3996122E-56.6301571
CTTCTTT23951.3649151E-76.41422751
CTTTTAT18400.0012184685.56596851
Produced by FastQC (version 0.11.5)