FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007915

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007915
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1191012
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTCA5250.001339987210.9969197
CTCACGA4900.00915661110.4402231
CTAAAAC6000.00366051569.7442071
CTCTCCT9001.3377555E-48.9321911
CTCCGCG7550.0024500278.7117091
CTCCAAG8608.383599E-48.4978551
CCTCGGG7850.00348606368.37877751
ATTGGTT10558.1607395E-58.3125941
CCCAGAT11502.4746767E-58.2613941
CGAAGAT14508.10247E-78.0641731
CCTCGAT13853.649744E-67.9149711
CTTCAGA10205.168748E-47.88134531
CTTTAAT16701.014123E-77.87705471
CTCAAAA15051.4217821E-67.7694681
TTCGAAT9550.00266446367.5580522
CTTCGAC12809.400854E-57.42234661
CTCTGAT9950.00352483157.34488061
CTTCGAT15051.1831793E-57.2838761
CTTCATT17451.7007314E-67.11969331
CTTCAAC13401.6546533E-47.0900021
Produced by FastQC (version 0.11.5)