FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007923

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007923
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences673212
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAATA3350.00997599112.94154552
CTCCTGA5651.2896486E-512.83365251
CCCCAAT4250.003361507311.9428471
CCCTGAT6253.629808E-511.6016231
CCCACAT4550.005593237511.1554061
CCTTTGG6550.0080302968.85621
CACCGAT6700.0096654848.6579271
CTCAAAA10457.9870355E-58.3265231
ACAATTT8950.00139058828.0734033
CTCATCC8100.00495780178.0566831
GTAGGGG1700.0056916687.9357185140-144
CATTTCA9400.00225420927.68691163
CAACAAT8500.0076395217.67754461
CTTTCAA12305.2562467E-47.07415961
CTTTATT12300.0040732246.48464631
CTTCCAT13300.0091758375.99707941
CTGGAAG13350.0095382795.97461841
CTTCTTT20358.325597E-45.3447281
Produced by FastQC (version 0.11.5)