FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007926

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007926
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences672351
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTGGAT4000.002071359212.7393581
ATCAACT5750.002655329610.1281861
AGTTGCA5950.0038864659.6727387
CTTCAAA12407.8968696E-78.8060081
CTTATAT8100.00478506238.0884821
CTTGTTT9850.00331341247.39049051
TGTAGAC2853.395401E-46.687062125-129
CTTTCAT12800.0059220356.2559351
CTTTTTT24706.502013E-55.30499651
TATATTT17850.00625852365.2401793
CTTTCTT18550.0085587535.10162971