FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007932

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007932
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences757432
Sequences flagged as poor quality0
Sequence length100-151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAC2003.5702006E-421.3022611
CCCGAAT5355.696511E-714.5996821
CCTCAAT5750.00326413929.879311
CTTCAGA8055.412846E-59.7028941
ATTGACG6000.00470575369.4514186
CTCCAAT6050.0049680239.3894271
CTCATCT7300.0023418638.75435451
ATCCGTT9202.3169396E-48.4900311
CTTGATT9352.7593263E-48.3538281
CTTTAAT15804.527965E-67.1906371
CTATAAT10900.00142389767.16589831
CTTCAAT14156.0153317E-57.0254811
CTTCTGT12306.680071E-46.9275651
CTTCCTT16801.1264548E-56.76262331
CTTTCAT16707.5257274E-56.3779231
CAAAAAG23108.2476254E-76.14783861
CAGATTT14200.00344612825.99033553
ATCAGAA14250.0035158985.9795831
ATTCATC15550.0014580375.92613032
CTTTTCA19258.2522696E-55.90192561
Produced by FastQC (version 0.11.5)