FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007933

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007933
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2937562
Sequences flagged as poor quality0
Sequence length100-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAAAT21953.934474E-97.27830551
CTCACCC17101.3701138E-67.21929741
CTCAATT14455.925249E-57.03561971
CTTTAAC15501.4835487E-46.55901241
CTCCCAA15851.9829853E-46.4141761
CAACGTT14000.002250066.22436951
CTCAAGA22201.8775208E-66.21502351
CTCTGAT16503.334553E-46.1614961
CCCACTT13400.0097895445.96114971
CCCGTAT19557.4970725E-55.9431321
CTCGATT15950.00148486025.9186791
CTTCTAT23605.317006E-65.8463361
CTTAAAT16150.00172347735.84538271
CTCGAAC21302.5701328E-45.45484641
CACCCGT16000.0099144445.44632341
CTTAAAA25902.5326215E-55.32716271
CCCAAAG20659.972975E-45.27488951
CCCAGAG17900.00582572865.27390671
CCCATCT24857.487789E-55.26003031
AACAAAC23451.9103385E-45.23821542
Produced by FastQC (version 0.11.5)