FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007943

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007943
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences792278
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCACGT1850.004777100419.7809541
GTCCAAT3250.007504276513.5119121
CTTTAAG6957.643837E-611.5839541
CTAATAT4650.00613622411.0177781
AATTTCG6859.566598E-510.5483555
CTCTTAT6050.003870399.6779541
CCCATCT6400.0061524369.1486911
CGAAACG6400.0068366779.0320293
CTCAAAA9903.8034697E-58.8714581
CTCTTGC6600.0079202548.8714581
CCCGTTC6700.0089583088.7390481
GTTTGAT10004.1085237E-48.0508481
CTCATAA8600.00780609257.6593691
CTTATTT11602.4098236E-47.5713311
CTCATTT8800.0095851337.4852921
CTCCATT9900.00330453677.39288141
CTTTATA13953.345346E-57.34518531
CATTGAT14103.8533763E-57.26704551
TCAAAGA15001.3222116E-57.22562362
CAATCAT11750.00228666936.85178571
Produced by FastQC (version 0.11.5)