FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007944

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007944
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1149196
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC5901.4551915E-1118.3549753
CCGATCT7057.794679E-712.2887214
CTTCCGA12456.657501E-1010.5635031
CTCCGCT7903.2210126E-510.1735011
CGTGAAT6506.2520313E-410.1165861
CTCGGAG6250.00513654859.35222151
CTCGGCT7150.00149658799.1968971
CTCCCAC6750.0096599858.6594651
GTCTGAT9402.1493742E-48.550071
TTCCGAT15352.9936928E-88.4660082
CTAAAAT12054.4518318E-57.8824481
CCCAGAA8350.0060904047.8751861
CTTCAAT20456.830305E-97.50292831
CTTCCAC16658.1289E-77.4600121
CTTTCAT20809.480573E-97.3766771
CTCATTG10000.00370992287.3064231
CATCGAT13001.1419935E-47.3064231
GTCAGAA10200.0044996567.163161
CCTTGAA15451.7159971E-57.09361461
CCCAAAA10400.00543448937.02540641
Produced by FastQC (version 0.11.5)