FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007946

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007946
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences431319
Sequences flagged as poor quality0
Sequence length100-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCATCTCGTATGCCGT9810.22744187017033796TruSeq Adapter, Index 18 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC2500.068.239948
CACACGT2650.064.37737
AGCACAC3450.049.4434935
GAGCACA3850.046.1526154
AGAGCAC3950.043.1748123
CACGTCT4000.042.649969
GCACACG4550.037.490126
GAAGAGC6100.028.0191761
AAGAGCA7450.022.8966522
AGTCACG2100.016.24383520-24
CAGTCAC2800.013.70573720-24
GTCACGT2750.012.40438520-24
ACTCCAG2950.012.04410115-19
TCTCGTA3000.011.84309135-39
CCAGTCA3350.011.45554120-24
CTCGTAT3300.010.76644535-39
TCACGTC3500.010.55751325-29
AAATACC5800.00347396249.8034526
CTCCAGT3850.09.59774115-19
TCCAGTC4150.09.24724320-24
Produced by FastQC (version 0.11.5)