FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007948

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007948
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences587840
Sequences flagged as poor quality0
Sequence length100-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCACAG2750.00262108815.8292241
CTTCGCG3200.00717437213.6032391
CTCAAAG7401.6881751E-510.784551
CTTTAAA12356.493792E-1010.5741781
CTCATAT6759.417406E-49.6734141
CAGAACA6150.0048792679.40934853
CATCACA6400.00660788559.0688261
CTCCATT7700.00312290018.479941
CTTCAAT13051.5475895E-57.7832071
CAGAATG8600.008664157.5698765
CTTTCAT10900.00113234197.32162051
CTTCAAA14053.2241314E-46.71286771
GTTCTTT12700.00565076856.28391081
CTTCCTT14550.00348618745.9835551
CATCAAT13350.0094786375.97795251
CTTCTTC21150.00138096835.14543341
Produced by FastQC (version 0.11.5)