FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007954

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007954
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2540915
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAAA8500.00114474849.471601145
CTTAGCC7200.00158290549.1413761
CTCAAAT22005.2750693E-117.9779281
CTCCGAT19251.7515049E-87.5980271
CCCCAGT10809.462859E-47.4485291
CTCAGAT14505.650687E-57.06092451
CCCCGAC16706.6931534E-67.0065631
CTTTAAT31801.8189894E-126.8991521
CTAAAGA14002.8127467E-46.79073671
CTCCGAG16504.251874E-56.64827351
CTCCCAT15107.057033E-46.2960471
CTCCGCT26953.0429874E-86.2412361
CTCCGTT23607.1885006E-76.1975431
GTCAGAT22502.0931184E-66.1755071
CTATAAT25003.1000855E-76.14300441
CATCGAT21556.8343797E-66.1083911
CTCGACG22902.827548E-66.0676381
CTTTGGG16904.144509E-46.05819031
CTTATTT32701.1768861E-96.038341
CTATTAT31603.4033292E-96.01710841
Produced by FastQC (version 0.11.5)