FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007966

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007966
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences691546
Sequences flagged as poor quality0
Sequence length100-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTAAT3609.872224E-414.0555381
CCCAGCT5200.00121907911.1208671
CTCAAAT6154.1392236E-410.5783841
CTCATTT7652.90221E-49.4491021
CTCATCG7050.00144975649.2279531
TCAATCC6350.00663142149.0653213
CTCCATG6450.00726125538.965661
CTTTGAA12105.346298E-57.76622441
CTTTTAA14306.692795E-67.58240941
CCTGCTT12608.839984E-57.4580411
CTTCTTT25452.910383E-117.38477951
CTTTTAC11300.00172583617.03665451
CTTTGAT10800.0086753976.69311431
ATTGATT12700.00587352376.260961
ATATTCT17256.6072645E-45.8399066
Produced by FastQC (version 0.11.5)