FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007967

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007967
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1140061
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCAAT5400.001515374610.8359241
CTCAAAT9251.5451151E-610.2794861
TCGATTC7300.00200367078.9067933
CTTTCGA8356.199557E-48.759581
CTTATAT11452.3183331E-58.3043881
CCCGCTT11803.3806566E-58.0580711
CAACAAA17903.8884536E-87.76372861
CTCAAAA15001.11564805E-57.314251
CATCCGT13802.3500135E-46.89023541
CAACAAG13500.00137511386.5015551
CTAAAAT12400.0040542956.48844671
CATCTGT12500.00440913356.43653961
CTTTGAA17851.2543239E-46.14642861
CTTTGAT19104.8028385E-56.127121
CATCAAT18051.4613841E-46.0783241
CCTGCAT17255.3709216E-45.93620251
CTTTTAA18009.2468044E-45.6888611
CTTTCTT26102.5444408E-55.32454971
CTTCTTT33454.1949716E-75.24789241
CTTTCCG23952.1763239E-45.1917431
Produced by FastQC (version 0.11.5)