FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007975

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007975
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1139489
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCAAT7903.3658944E-510.1328951
CTCCGAA9803.4509558E-69.6535091
CTCCGAT8558.009707E-59.3625581
CTTGGAT6450.00687764039.026061
CTTCGAC10054.858628E-58.6892671
CCCGTTT9452.3769018E-48.4708861
CCTCAAG8000.0042959668.1869191
CTCTATC9200.00170338297.91006661
CTTCTAT13502.3403943E-57.54678961
ATTTGCG9700.00321875637.4134456
CTTCATT15602.077973E-56.99736641
CTTTGGT10600.0067948836.86534071
CTCCGCT10650.0071114346.8331091
CTATCAT10750.00778381766.76954561
CTTTTAT19902.011293E-66.58244751
CAGATTT16907.455699E-56.38314153
CTGATAG12550.00484630936.3784761
CTTTAAT12650.00526410156.32805351
GCATATA12750.00646121666.20458034
CTCAAAA15459.856808E-46.1232621
Produced by FastQC (version 0.11.5)