FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007980

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007980
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences954671
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC2806.7894016E-620.4555428
CACACGT2857.912151E-620.0966727
CACGTCT3851.0473423E-414.8744199
CTCAGGG4200.002928539912.1656431
GCACACG6003.6035833E-410.7391596
GAGCACA6154.5264862E-410.4772294
ATCCAAT7752.6342323E-510.3604191
CTCAGAA8204.9006998E-59.7918591
AGCACAC5900.00377471939.7077145
AGAGCAC6709.946278E-49.6171583
TAGAGAT6550.0089169138.7443533
AATTCTC9703.7549218E-48.1189525
CTTGGAA11351.9335681E-47.71741251
CAATAAA15251.7640323E-67.6583721
TAGATTT9350.00234388587.65715463
CAACAAC13452.138273E-57.59787371
CTTCAAT12809.534399E-57.4134391
CTTTAAA11250.00148673687.13717751
TTAAAGA12205.5440003E-47.0420722
CTCAAAG10650.0069030466.85388331
Produced by FastQC (version 0.11.5)