FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007997

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007997
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1244480
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTACAT6600.00884873058.7529321
CTTTGAG13954.793652E-67.7646971
CCCTGCT12457.735179E-57.54017541
TGCGATA9600.00286818067.50146872
CTAATTT10750.0083890346.71736571
CTTTAAC11850.00288305576.7031941
GTTAAAG12000.0032897846.6194041
CTTCTAT15007.578131E-46.2583461
CTTTTAT27654.1861495E-75.74559451
CTGTAAA16400.00220978125.7240971
ATCGGAA17000.0033847265.522071
CTGAAAT18500.00153953545.46466731