FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007999

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007999
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences655472
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTCGC3905.1596908E-616.8771931
CCCATGG4251.1669943E-515.4873061
CTCATGA3300.00834059713.2971821
CTTACAT4600.005691828711.1291641
CCTTTAC7401.5455444E-510.8713451
CTTCAAT10356.062801E-79.8925891
CTTCGAT7803.1360536E-49.3762181
CTTCTAT6550.00748465168.9324591
CTTTCCT9000.00130393038.1260551
CTTTTTA12809.2944545E-57.42772251
CTTTGTT11853.1055207E-47.40602541
CTTTGAT10150.0042435497.20536951
CTTCCGG13551.8760667E-47.0165941
CTTCCAT11800.00240866076.8176231
CTTTCAT11850.0025182576.78885651
TTCAATA11750.00261914756.7635782
CTTTTCT14650.00344266845.9905391
CTTCTTT19808.0848215E-55.90985871
GGACTGT3803.4838272E-45.70390995-99
Produced by FastQC (version 0.11.5)