FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008007

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008007
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences997071
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAGGA1750.003661326620.7117351
CTCACAT4109.035757E-615.9126731
CTTCCGT14207.185008E-109.6995091
CTCTGAA7100.00150799459.1890091
TCCAACA9201.9279082E-48.6361117
CTTTGAT14501.00986654E-78.4989531
CTTCAGA9402.3397409E-48.4829981
CTTTGGA11151.8575678E-58.4518741
CAATCTC8150.00542315387.9774424
CTTTTAC14601.0155381E-67.94422671
CTCCTGT10951.3824584E-47.94422671
CTTCACG10054.8082374E-47.93434521
CCCGAAT8550.0080766747.6306381
CTTTTAT18306.7198926E-87.52639441
CCCAGAT8700.0094335997.4990761
CGAAAGG8750.0099281967.4562241
CCCAAAA11753.1235124E-47.40334271
CTCCGCT10050.00420452937.2130411
CCCTTTT14255.0335046E-57.1219291
CTGAAAT16305.3018994E-67.11568741
Produced by FastQC (version 0.11.5)