FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008008

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008008
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3263059
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCT12609.17836E-68.0970741
CTCAGAA28550.07.91272741
CTTTAAT46550.07.82746121
CTCTAAT14507.302204E-67.53865531
CCCAAAT20709.131327E-97.39298061
CTCCGAT26054.1836756E-117.2733791
CCCATTA13101.2989968E-47.23173761
CTTAGAT11100.00131479657.2217141
CGTAGAC11200.00144593677.1572351
GGCCCTC10400.00615057356.9371849
CTACCGA15009.237185E-56.8015421
CTCATTA21601.5478145E-76.74756151
CTCTCGA14053.0683118E-46.74275881
CCCAGAT30704.1836756E-116.6464581
CTCAACT17901.9673875E-56.5138471
CCCATAT25105.3090844E-86.3873331
CTTAAAG27406.859409E-96.38309431
GTCCAAG16002.1409063E-46.37644531
CTCAAAA46100.06.3230941
CCCACTT17308.600626E-56.3185261
Produced by FastQC (version 0.11.5)