FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008009

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008009
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2121077
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTCAT11954.1573003E-89.7921341
CTTACAT12508.324423E-89.361281
CCCTCAG6550.0074958838.9325191
CCCCTGT8255.508582E-48.8648481
CTTACGA9954.0811137E-58.8203011
CCCACGA10004.3285418E-58.7761991
CTCACAG8406.5920275E-48.7065481
CTCACAA9301.8963656E-48.6503761
CTTATAC16156.2609615E-98.6041171
CTCCTAT13101.6340764E-68.3742361
CTTAAAG19252.3646862E-108.3582861
CTCCCTT14508.029165E-78.0700681
CCCGTCT11803.38995E-58.0572451
CTTGGAT12801.0793963E-57.99914071
CTCAGCT11001.3174329E-47.9783631
CTTCGAT20701.0404619E-97.77280141
CTTTGAT33000.07.7567421
CCCCCAC8650.0082878297.60942171
GTCCATT14557.282406E-67.53969051
CTCCCGT16557.2926014E-77.51235631
Produced by FastQC (version 0.11.5)