FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008019

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008019
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4267182
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCACAT28701.8189894E-127.36551951
CTTCAAT65100.07.05418541
CTCTAAT13451.7918204E-47.0454441
CTCAGTT18908.3982377E-76.94221451
CTCTCAT23101.0164513E-86.9422141
CTCAGCT23551.0706208E-76.5000351
CCCGTGA26452.219167E-86.33854341
CTTAAAT32651.891749E-106.25118161
CTTATTT60350.06.1599931
CCCCCAA18051.5356146E-46.05761051
CTTTGAA65350.05.9117711
CTCAGAT19909.178579E-55.86076351
CTTTTAT75300.05.8082271
CCCCAAT22851.8554394E-55.7421381
CCCACCT24157.3665487E-65.7348731
CTCCAAT33101.05483196E-85.7257541
CTCATAG30657.348535E-85.7077911
CTTAGGA15350.006021635.6984951
CTCACAT16650.0023701235.69136521
CCCAGGA28305.498132E-75.6666131
Produced by FastQC (version 0.11.5)