FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008025

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008025
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1084136
Sequences flagged as poor quality0
Sequence length100-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAGAT5701.4013451E-512.7321021
CTCATAT12307.2943294E-89.4403881
CCCAAAT7803.3927048E-49.3042291
CCTCGAT7954.105634E-49.1286771
CCCAGCT8255.9430895E-48.7967251
CTCCGCT8306.3118676E-48.7437331
CTCTGTT11854.2431493E-68.5740231
CTCAAAT9402.311827E-48.4925831
GGCTATT7900.00393132738.2678081
CTTAATT7950.0041614488.215811
CTCCTAT8000.0044033018.1644611
CTTCTAT14256.917726E-78.1485451
CTTAAAG10255.868876E-47.78832051
CTTTATT21954.965841E-107.6044591
CCTCAAT9700.0029422737.4817511
CTCTCTT10709.2862995E-47.4607741
CTTCATT19602.8026989E-87.40540651
CCCAGCA10800.00102529837.39169261
CTTTTAC16709.483756E-77.3876691
CTTTTAT21451.951048E-87.1050471