FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008026

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008026
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1157709
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAAAT8603.3651304E-1013.311581
CTCCCCA4650.007265454610.7709281
CTCAATT6659.343126E-49.6834221
CTCCCGA6850.00122527429.4006961
CTTAAAG10156.6539884E-58.4590831
CTTTTAT21555.4569682E-117.9684161
CTCCAAA11754.2213203E-57.9161411
CAATCAT12801.44403875E-57.82575231
CATAGAA12257.091352E-57.5930331
CTACAAT9600.00305522057.45309731
CTCCGGT9750.0035550517.33843471
CTTTAAA15653.5133944E-67.31498961
CTCACCG11753.631579E-47.3072071
CTCAAGG10900.00131507537.2206161
CTCCTCG12406.726517E-46.9241681
GATCAGC14901.1306275E-46.69608077
CTCAAAA15251.4529421E-46.56851
TAATATA15201.4629192E-46.56505544
CTTCAAT17451.766692E-56.5604341
CCTTGCT12250.00449364736.4248741
Produced by FastQC (version 0.11.5)