FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008028

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008028
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences624154
Sequences flagged as poor quality0
Sequence length100-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTCT2900.040.9385459
CACACGT3050.038.925177
ACACGTC3650.032.5265128
AGAGCAC5150.024.4088173
GAGCACA4950.023.9841964
AGCACAC5300.023.7161035
GCACACG6050.020.7760876
CCCGTAC2750.00333129915.2700551
GAAGAGC8451.8189894E-1214.9086341
AAGAGCA8953.6379788E-1214.0464232
AGTCACC2353.6379788E-1211.88417920-24
CTTAACA2705.693437E-109.827430-34
CTTGAGC6408.206516E-49.8199486
GCTTAAC2902.0372681E-109.63120930-34
CTCGTAT2705.9062586E-99.311810535-39
CCAGTCA3353.3833203E-108.75349620-24
CAGTCAC3404.5292836E-108.62476920-24
CTCCAGT4001.5825208E-108.02924915-19
ACTCCAG4153.43789E-107.739034715-19
TCGTATG3651.537228E-87.65207140-44
Produced by FastQC (version 0.11.5)