FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008029

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008029
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1372410
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTTAT11904.0272425E-910.3587161
CTTCATT21350.09.8492711
CCTCTGA6650.0091001838.7231291
CCCAAGC7800.00353270198.3666541
CTTCAAT19654.274625E-108.1182811
CTCAATT8150.0052463418.0073511
CACAGTT9200.0017654847.88163141
CTCGTTC11352.089932E-47.6663621
CTTTTGG18105.5208147E-87.6116531
CTCCAGT8600.0084918377.58836131
CTTAAAC8650.0089437.5444981
CCTCTGT11350.00175291067.02749821
CTTTAAT18808.365205E-76.94254351
CCCTGAT11600.00220607766.8760441
CTTTAAA20203.7715108E-76.82034251
CCCATTT14103.400722E-46.68541241
CATCAAT24453.475543E-86.5245081
CTCAAAG14956.925103E-46.3053051
CTTGATT16553.5282184E-46.13386151
CTTTCGT13100.0078584896.08871031
Produced by FastQC (version 0.11.5)