FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008039

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008039
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1131126
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAATG4652.055458E-1020.2689341
TAGTCTA2353.0377123E-1011.05243385-89
CTAATGA9953.6543497E-810.8770382
GCCCCCC2909.094947E-1210.47650935-39
CCCGGGA7101.3301148E-410.2113271
CCAAGAT6850.00108594549.5256031
CAACTAA6600.0089249448.7436457
ATTGTTA15107.359851E-57.569885145
AACTGCC11452.4522154E-47.56067569
CCCCCCG2702.0785458E-46.960925640-44
AGTCTAA3952.792549E-76.94080385-89
AAACTGC17702.610468E-66.92822658
GAAACTG18756.424212E-66.5402467
ATGAAAC20052.6282087E-66.4759665
AGCCCCC5005.6828867E-86.36572635-39
AGATGCT14050.00251994076.1610026
TGAAACT23052.512792E-55.63310676
CTTCAAA19554.8774589E-45.5626921
ATCGAAA17300.00396907475.44800851
GTCTAAT6402.2412496E-75.41108785-89
Produced by FastQC (version 0.11.5)