FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008046

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008046
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences674305
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTACGT1358.2438573E-426.7596916
CTCACGG5651.6972308E-411.6463971
CCCATAT5550.001908487810.5388821
TCCAAGA4850.00923969410.4264562
CTTTAAC5850.00295492879.9984271
CTATTCC6000.00364379459.7484661
CTTTCGC6150.00446805739.5106981
TCAAAGG7000.00136630279.2880582
CTTCACG6750.0095982668.6653031
CTCCAGA7950.00388813048.2771
TTACATT8750.00111300858.2572774
ATTCAAT9000.00130775928.1237221
AGTCACA2100.00667813656.878512450-54
CTTTCTT20951.8303662E-45.5838471
Produced by FastQC (version 0.11.5)