FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008050

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008050
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3972822
Sequences flagged as poor quality0
Sequence length100-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCACAT13151.792583E-57.7012151
TAGATCT11100.00142686557.1663073
CTCAAAC32650.06.8680811
CTTCGAT41400.06.81429961
CTTTAAT44750.06.62746951
CTCAATT24052.6409907E-86.61705161
CTTCAAT55300.06.4095551
CTCCATT36903.6379788E-126.27307651
CTAAGAA16653.9399814E-46.08234071
CTCTGAT26651.9887557E-75.97148561
CTCAGTT17105.548077E-45.9222791
CCCTCTT26952.473207E-75.9050131
GTCAGAG23404.9189075E-65.87346941
CTTCGAA23555.481046E-65.83605861
CTTAAAT28601.2763849E-75.81726361
CTTCAAA67250.05.80842541
CTCAGGA25102.6234593E-65.76385741
CTCAAAT31502.5409463E-85.7409851
CTTTGAC52950.05.7377321
CTTCATT59600.05.70438151
Produced by FastQC (version 0.11.5)