FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008056

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008056
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1824294
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGAAAC21001.4464604E-87.2177741
CTCAAAC12255.3023966E-47.07047221
CTTTCAC18002.455173E-56.41579871
CTAAAAT23055.9506056E-76.262711
CCCGCAT14550.00370437055.9528031
CTTTAAT28053.3367487E-65.4036811
CCAGAAT23652.2021182E-45.18825151
CTTTCAT34601.1068405E-65.0065481
Produced by FastQC (version 0.11.5)