FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008067

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008067
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences690192
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCACAT3203.9608125E-415.8539011
TTTAACG4601.5969508E-615.7017427
CTCAGAT3859.418543E-515.0597361
ATTAACG3200.007390795313.5427536
CCTCGTT5408.130677E-613.4212911
CTCAATT4000.002141505512.6831211
CTCATGG5351.1078754E-412.1920521
CTCCGAT6857.2521034E-611.6383171
TTTTAAC7551.7690818E-611.4799496
CTCCCTT5150.001100138211.2582481
CCCAAAT5300.001398414610.9396181
CCCAGTC4700.007145224610.7941461
CTTAAAG5400.001634350210.7370341
CAACAGT6405.833931E-410.1917931
CTTCAAT12105.7672878E-89.58346751
CTTATAT7803.4349042E-49.2916631
CTCCATT9452.4671665E-59.2031721
CCCATTT7200.00171499839.0593711
CCCAAAA7250.00182649468.9968941
CTCCAGA6650.0091276948.7187941
Produced by FastQC (version 0.11.5)