FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008069

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008069
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2198638
Sequences flagged as poor quality0
Sequence length100-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCAAAT9151.3598819E-610.3835951
CTCTGAT15753.6379788E-1210.2086321
CTAATAT9903.725254E-69.5969591
CCCCGAT12357.073031E-78.8766631
CACACGG9201.6993826E-48.7383681
CTCTGGA20156.91216E-118.34215451
CCAAGAT12951.2729806E-57.9010311
CTTTCGA13201.6427122E-57.751391
CTTCGAT24801.2732926E-117.6620891
CTTTAAA28800.07.6129721
CTCCGAT14758.918905E-67.43232541
CTTTTAT33750.07.36259031
CTCACGG12154.185069E-47.21822551
TAAACCA17101.4963298E-67.1787974
CTCAGAT11250.00146955117.1460441
CTTCAAA29351.2732926E-116.9722891
CTCAAAT13702.1737015E-46.93502861
CAACAAT21109.570795E-86.92744451
CTCAAAG20052.8945578E-76.92571641
CTAAAAT16908.060333E-66.91924571
Produced by FastQC (version 0.11.5)