FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008072

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008072
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1693828
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC12951.4733814E-1010.5827696
CCGATCT14801.6189006E-109.7477
CTACAGA8356.8981154E-48.6667161
CTTGGAT11201.834272E-47.7536161
ATCGAGA12608.743767E-57.46644451
CTTCCGA20307.605195E-97.46237754
CAATTCA15803.5914472E-67.30494364
GCTCTTC21852.9438525E-86.95518881
ACGATCT13000.00105011156.6587373
GAATTCA18051.7686325E-45.99470764
AATTCAA24651.9972795E-65.8523275
TTTCACA21053.8341397E-55.8250487
TTCCGAT24852.305358E-65.8052265
CTCTTCC29905.406946E-85.79071952
TCACATT15400.00699186235.62150962
CAAACAT17150.00366336435.48555141
CTCAAAA21653.400513E-45.34814451
Produced by FastQC (version 0.11.5)