FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008075

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008075
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6170492
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCAAAT39050.06.78380251
CCCTGAG23305.1192546E-76.31634431
CTTCGAT62300.06.141961
CTTCAAT94150.05.70550731
CTCCGAA54700.05.65006831
CTCAAAG64250.05.61196141
CTCAAGT21252.0589486E-45.5405491
CTCAGTT26002.1687023E-55.3773951
CTCAAAT49307.2759576E-125.37337641
CCCACGT17850.0048283135.35916141
GTCCAAT30652.1189317E-65.28182361
CACACGA19600.00251747325.2561011
CCCAAGG36658.405368E-85.22024731
CTCGTGA21150.00115089885.21882341
CCCAAAA66450.05.20468661
CCCTAAA20350.0040232045.0623871
CTTAAAT52404.1836756E-115.0554861
CTTAAAA75900.05.04142481
CTAAGAT21950.00188400225.02861551
Produced by FastQC (version 0.11.5)