FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008084

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008084
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2571524
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCTAT14851.3326917E-67.80414961
CCTATCG9500.0024708557.61718236
CTTCCGA20503.5437188E-66.35990671
CCACAAT20652.6838115E-55.9629471
CCCATTT24751.2373195E-55.56045681
ATTTGCG22601.0731149E-45.4432526
GTTTGAT31357.8275116E-75.314011
CCCGGCT21853.828949E-45.30396461
CCTCAAT19800.00347915975.1214741
GTCAAAG28502.3657247E-55.0829661
Produced by FastQC (version 0.11.5)