FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008095

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008095
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences730617
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCACCG6850.00106506079.5449061
TCACATT7550.00270696578.6154922
CTTTAAA11051.6119986E-58.5467451
CTTAAAA8709.963721E-48.3502681
CTTCCAT11502.6616566E-58.2123071
CTTATTT11101.5771232E-47.8537661
CTTTCTT17101.6630838E-77.64708761
CTCAAAA11502.3753679E-47.58059171
CTTTAAT10500.00629686336.9187941
TTAATAA12850.0066474876.1869143
CTCTTTT15000.00482583935.81178671
Produced by FastQC (version 0.11.5)