FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008098

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008098
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1605130
Sequences flagged as poor quality0
Sequence length100-151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTGT3804.5271845E-617.1139531
CTCAGTA6600.0088038428.7586561
CTTGGAC7500.00255717628.6710691
CTCCAAT15403.122659E-88.4458471
CTCCGAT12257.0389433E-68.2581621
ACCTCTC8750.00112357838.2509572
CCCAGCT9150.00173201537.89714861
CTTCAAT31200.07.8743691
CTCAAAC13804.0766718E-67.85422951
CTCTTAA12305.483085E-47.04964971
CCCAAAG10300.00551294267.01542851
CTTAAAA27953.6379788E-116.98028851
CTCTTAT17852.9051298E-66.8818011
CCCTGAT13852.8530092E-46.7824251
CTCAAGG10900.00953144956.6292581
CTTCGAG12000.0032687776.6237341
CTTCAAC21351.0440126E-66.4305361
CTTCCCA12400.00460588756.41006471
CACATTT17158.560899E-56.3200211
CTCATCG17208.911399E-56.3016491
Produced by FastQC (version 0.11.5)