FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008099

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008099
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences656088
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC3106.4185244E-414.8776798
CACACGT2700.00440389814.642647
GTTAATA2900.00704118813.6421612
TTATGTG3650.002200996712.63687
CTAATCT2501.7710289E-411.773939145
AGCACAC4750.001240488811.0975815
GCACACG4350.00815627110.6032916
AAAATTG5800.00649283979.0885355
ACTAATC2550.0023344958.758703140-144
GAGCACA6050.0091767538.7129764
GGCACTA3154.7394572E-47.945922135-139
CTTTTAT7650.00664700437.797751
TTTATTT9400.00554174327.0103093