FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008115

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008115
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1999754
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATCGA8300.00649706747.81921154
GTCGGAT10750.0081382566.7389581
CTTGGAG13000.00100089096.68711951
CCCTTGT14153.593947E-46.6556141
CTCAAAT16906.751279E-56.4299231
CGACAAT15158.2290074E-46.2162991
ATTGAGC13100.0083287246.0549296
CCCAGCT20752.8811282E-55.9351551
CTTCGGT14700.00398656455.91377931
CCCCGAA14800.0042990365.87382131
CTGATCC17356.945625E-45.8187153
CAAGCGG16850.00293671835.58913561
GTTCGAT15850.0091743875.4847041
CCCTGAT18950.00200364025.3520481
Produced by FastQC (version 0.11.5)